1.Retrieval and Storage of Protein Crystal Samples: Gently retrieve protein crystal samples using a pin tool and place them in a puck. Ensure that the puck container is stored in a liquid nitrogen environment to maintain sample stability.

2.Base Selection Recommendation: It is recommended to use SPINE-type bases, ensuring the pin length is 18mm. Additionally, the vertical height from the base bottom to the sample loop should remain at 22mm.

3.Preparation of Non-Protein Crystal Samples: For non-protein crystal samples, it is recommended to contact the user responsible in advance to discuss and confirm the specific details of sample preparation.