The functional group quantification analysis of aquatic green algae was studied by Nanjing Institute of Geography and Limnology. The toxic effects of Cd on Cosmarium sp. cells were characterized in vivo using SRIR microspectroscopy. The mapping and functional group quantification of biological macromolecules were conducted within a single algal cell. The results showed that the biosynthesis of biological macromolecules, such as carbohydrates, proteins, and lipids, in algal cells, is inhibited by heavy metals. Additionally, correlation analysis and principal component analysis show a good correlation between ν (C-OH)/Amide II and biochemical parameters of algae cells. These results indicate that carbohydrate changes in algal cells can serve as biomarkers of the toxic effects of heavy metals on single-cell microalgae. (Science of the Total Environment, 2023, 870: 161870)
Fig. SRIR imaging of aquatic green algae with the cytotoxicity of cadmium and Mathematical analysis
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