BL10U2

Home BeamlinesSSRFBeamline MapsBL10U2Beamline introduction
Scientific Scope
More

As part of the SSRF Phase-II project launched in December 2016, BSL-2 Macromolecular Crystallography Beamline(BL10U2) was established as China's first MX beamline with a biosafety protection function, facilitating the acquisition of structural data on pathogens by structural biologists. BL10U2 is aimed at a broad range of moderate-risk infectious viruses that are present in the community and associated with human disease of varying severity. With P2 level protection, the users can carry out the diffraction experiment directly from the active virus particle crystal. Resolving the crystal structure and functionality of viral particles, aiming to develop novel, efficient, and specific detection methods, vaccines, and therapeutic drugs.

Beamline Layout
More
Specifications
More
 项目   Designed specifications  Acceptance specifications  Measured values
 Energy range   7~18 keV  7~18 keV  6.5~18.1 keV
 Energy resolution   2×10-4  2×10-4  (1.78±0.02)×10-4
 Flux at 12.7 keV@300 mA,focus at sample   2×1012 phs/s  1×1012 phs/s  (2.52±0.01)×1012 phs/s
 Beam size options (@12.7keV)   20.0×10.0 m2  30.0×15.0 m2  (16.8±0.1)×(9.4±0.6) m2
 Beam divergence (@12.7keV)   1.5×0.2 mrad2  2.0×0.5 mrad2  (0.411±0.003)×(0.187±0.006) mrad2
 P2 level biosafety protection   Yes  Yes  Yes
 Guide to samples screened   >15 samples/hour  >15 samples/hour  34 samples/hour
 Highest diffraction Wavelength   ≤0.800 Å  ≤0.800 Å  0.673 Å
Endstations
More
The end station is currently equipped with a large active area detector (Eiger X 16 M) along with two high-precision diffractometers developed in-house, which can be switched to perform conventional cryo-crystal diffraction experiments or in situ crystal diffraction experiments. Additionally, an in-house-developed sample mounting robot enables fully automated rapid sample exchange in less than 30 s. Data collection from the diffractometer and detector is controlled by an in-house-developed data collection software (Finback) with a user-friendly interface and convenient operation. 
　　Fig 1 Endstations
（1）Detector
BSL-2 Macromolecular Crystallography Beamline(BL10U2) is equipped with a large active area detector (Eiger X 16M). Currently, major data processing software (including iMosflm, XDS, DIALS, autoPX, HKL2000 etc.) directly support the experimental data of Eiger X 16M, and there is no need to decompress the data before processing. Unlike traditional detectors, the Eiger detector collects raw diffraction data in a file format called HDF5 instead of one file per diffraction pattern. HDF5 is a popular and open-source container format for storing scientific data. Currently widely used in research fields including artificial intelligence and free electron lasers. There are two types of HDF5 files output by Eiger X 16M, namely master files and data files. Among them, the master file mainly stores metadata, similar to an image header, but provides much more information, and can also save a small amount of original diffraction data. The data file saves the original diffraction data. When viewing diffraction patterns or data processing, simply open the master file to access the entire set of data (except for Adxv, which requires opening the data file).
　　Tab 1 Specifications of Eiger X 16M
Eiger X 16M
 Pixel size   75×75 µm2
 Pixel count   4150×4371
 Effective area  311.2×327.8 mm2
 Maximum speed  1 mm/s
 Movement distance range from Detector sample to point  135-800 mm
 Read frame rate
 133 Hz（Full）
 750 Hz（4 M）
 Maximum resolution  0.685 Å
 
（2）In-house-developed two high-precision diffractometers
During the construction of the experimental station, we overcame the engineering difficulties caused by the scientific need for radiation protection and biosafety to coexist. We independently designed and developed two high-precision diffractometers which can be switched to perform conventional cryo-crystal diffraction experiments or in situ crystal diffraction experiments.
Fig 2 In situ crystal diffraction experiments
　　Fig 3 Conventional cryo-crystal diffraction experiments
（3）In-house-developed sample Changer
Samples can be mounted either manually or using an in-house-developed sample changer called swordfish, which requires approximately 20 s to remove a crystal sample from the goniometer and replace it with a new one in the storage Dewar capable of storing 320 samples (20 pucks) at a time. A barcode reader was used to scan the crystal information, which was useful for recording information during high-throughput crystal screening. A standard pin with a length of 18 mm is recommended. Routine calibration and maintenance ensure reliability, and an increasing number of crystals have been easily screened since the installation of swordfish.
Fig 4 Design drawings of in-house-developed sample Changer (A. Physical diagram B. Overall composition diagram C. Design diagram of new storage Dewar D. Design diagram of new robot gripper)
Data Acquisition and Analysis
More
1.Single crystal
2.MAD/SAD
3.MIR
4.MR
5.Shutter-less data collection
6.in situ data collection
7.BSL-2 MX experiments