Infrared spectroscopy (including far infrared) covers the vibration, rotation and energy transition of molecular interactions of biological macromolecules (such as proteins, nucleic acids, sugars, lipids, etc.), and therefore becomes a powerful tool for analyzing the chemical composition of biological samples. When using high-brightness synchrotron radiation for infrared microscopy and imaging research, high spatial resolution of the theoretical diffraction limit can be achieved, and infrared spectra with high intensity and signal-to-noise ratio can still be obtained when the aperture size is 5μm×5μm, overcoming the shortcomings of the traditional FTIR spectrometer light source with weak signal, poor signal-to-noise ratio and low spatial resolution under the small aperture. 

 

Synchrotron radiation infrared spectroscopy can be applied to the analysis of many types of samples in biomedicine, pharmaceutical chemistry, chemistry, materials, environmental science and other fields. Meanwhile, the infrared time-resolved experimental station can detect the physical changes of structure and spatial morphology during the dynamic process of sample points. The time scale of the infrared spectral transition is picosecond, which is much smaller than that of nuclear magnetic resonance analysis, so it has the potential to study the time-resolved molecular structure dynamics.

The BL01B time-resolved and infrared microspectroscopy endstation combines high brightness synchrotron radiation (SR) infrared light, Nicolet FTIR infrared infrared spectrometer, Continuμm infrared microscope, Bruker Vertex 70V vacuum infrared spectrometer, Hyperion II Infrared microscope, high sensitivity MCT detector for high spatial resolution infrared microscopy and mapping imaging research. The experimental endstation is equipped with ATR, Linkam heating and freezing stage, Leica freezing microtome, Leica vibratome, paraffin slicing machine, far-infrared Bolometer detectors and time-resolved step scanning systems. The endstation has developed the microfluidic infrared equipment and the in situ temperature-controlling system for drawing.

 

ThermoFisher-Nicolet IR microspectroscopy endstation

 

At the spatial scale, the BL01B endstation can perform high spatial resolution infrared spectroscopic microscopic analysis and point-by-point mapping scanning imaging studies on many types of samples (powders, films, cells, tissue sections, liquids, etc.). On a time scale, the time-resolved endstation can perform nanosecond (step scan) or millisecond (rapid scan) time-resolved studies.

 

Bruker IR endstation

 

Time-resolved IR endstation

The experimental data of Nicolet infrared spectrometer is automatically collected by OMNIC program, and the experimental data of Bruker infrared spectrometer is automatically collected by OPUS program. The sample position and range are selected by the microscope system, and are automatically scanned by the software control system. Data processing can be carried out using OMNIC or OPUS, and Cytospec software is also installed on the endstation. You can also refer to the following data processing software: