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BL17B1 High-throughput Protein Crystallography Beamline

Scientific goals

With the development of protein expression and crystallization technology, the efficiency of obtaining protein crystals is increasing, and the requirements for measuring the crystal structure of proteins are becoming higher and higher. In addition to macromolecular protein crystals, high-throughput protein crystallography beamline (BL17B1) can also be used by small molecule crystallography users in the field of chemistry and materials science.

Techniques and methods

Single-wavelength anomalous diffraction (SAD), Multi-wavelength anomalous diffraction (MAD), Molecular replacement (MR), (multiple or single) isomorphous replacement (MIR/SIR), Patterson function, direct methods, X-ray single crystal diffraction.

Beamline Layout

 

Beamline specification

Photon Energy range

5-20 keV

Wavelength range

0.62-2.48 ？

Energy resolution (DE/E)

￡3×10-4

Focused Beam size (FWHM) (H×V) (@300mA)

￡150×80 mm2 (@12keV)

Flux at sample (@300mA)

33′1011 phs/s (@12keV)

Focused beam divergence (H×V) (@300mA)

￡1.5×0.2mrad2

Endstation

Equipment

Type

Capacity

Diffractometer

ARINAX MD2

SOC< 1μm

（with Mini Kappa）

Detector

Rayonix MX300

300 mm×300mm

73μm×73μm/pixel

readout speed: 1s

Cryosystem

LN2 Oxford Cryosystem 700

80-400K

Sample mounting robot

Irelec CATS

storage capacity:

 180 samples

Distance between sample and detector

90-800mm

Data processing software

HKL3000, APEX3, Olex2, etc.